Installation
Prerequisite software
- Mash >= v2.0
- ~~SQLite3~~ Removed in version 1.4
- Perl
- multithreading
- BioPerl library
- ~~
DBD::SQLite~~ Removed in version 1.4
- Quicktree
Environment
Environment variables
For most Linux OSs, you will need to set up your environment like this:
export PATH=$HOME/bin:$PATH
export PERL5LIB=$PERL5LIB:$HOME/lib/perl5
System packages
Some system packages are needed. On Ubuntu, this is how you might install these packages.
apt update
apt install -y build-essential cpanminus libexpat1-dev wget
Perl packages
There are some perl packages too and so this is how you would install those on most Linux OSs:
cpanm -l ~ --notest BioPerl Bio::Sketch::Mash
Quicktree
mkdir -pv $HOME/bin/build
cd $HOME/bin/build
wget https://github.com/khowe/quicktree/archive/v2.5.tar.gz
tar xvf v2.5.tar.gz
cd quicktree-2.5
make
mv quicktree $HOME/bin/
Mash
mkdir -pv $HOME/bin/build
cd $HOME/bin/build
wget https://github.com/marbl/Mash/releases/download/v2.2/mash-Linux64-v2.2.tar
tar xvf mash-Linux64-v2.2.tar
mv -v mash-Linux64-v2.2/mash $HOME/bin/
Mashtree Installation from CPAN
Installing from CPAN installs the latest stable version of Mashtree. This method adds the Mashtree perl modules to the correct place in your home directory and adds the executables to your home bin directory.
export PERL5LIB=$PERL5LIB:$HOME/lib/perl5
cpanm -l ~ Mashtree
mashtree --help # verify it shows usage and not an error
Uninstallation from CPAN
cpanm --uninstall Mashtree --local-lib=$HOME
Other sources for Mashtree
Other instances of Mashtree can be found in the wild. Although I did not create these, others have found them useful. I cannot provide support for these outside instances.
Docker
- https://hub.docker.com/r/bioedge/mashtree
- https://hub.docker.com/r/staphb/mashtree
Conda
- https://anaconda.org/bioconda/mashtree