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Bio-Tools-Primer3Redux-0.09
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NAME

Bio::Tools::Primer3Redux::PrimerPair

SYNOPSIS

# get the primer pair from the Bio::Tools::Primer3Redux::Result
my $pair = $result->next_primer_pair;

# grab left/right primers
my ($fp, $rp) = ($pair->forward_primer, $pair->reverse_primer);

DESCRIPTION

Bio::Tools::Primer3Redux::PrimerPair acts as a simple SeqFeature that bundles primer pair data together into one object. This object can be used to retrieve the amplicon sequence, the forward/reversion (left/right) primers, and any internal oligos. Furthermore, any primer information relative to the product is included as SeqFeature tags.

NAME

Bio::Tools::Primer3Redux::PrimerPair - Simple Decorator of a Bio::SeqFeature::Generic with convenience methods for retrieving left and right primers, internal oligos, and any amplicon-related information

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated.

bioperl-l@bioperl.org                  - General discussion
http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

Reporting Bugs

Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web:

http://bugzilla.open-bio.org/

AUTHOR - Chris Fields

Email cjfields at bioperl dot org

Describe contact details here

CONTRIBUTORS

Additional contributors names and emails here

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

left_primer

Title    : left_primer
Note     : Alias of forward_primer()

forward_primer

Title    : forward_primer
Usage    : $obj->forward_primer
Function : returns the forward (left) primer
Returns  : Bio::Tools::Primer3Redux::Primer
Args     : Optional Bio::Tools::Primer3Redux::Primer

right_primer

Title    : right_primer
Note     : alias of reverse_primer()

reverse_primer

Title    : reverse_primer
Usage    : $obj->reverse_primer
Function : returns the reverse (right) primer
Returns  : Bio::Tools::Primer3Redux::Primer
Args     : Optional Bio::Tools::Primer3Redux::Primer

internal_oligo

Title    : internal_oligo
Usage    : $obj->internal_oligo
Function : returns the internal oligo (if present)
Returns  : Bio::Tools::Primer3Redux::Primer
Args     : Optional Bio::Tools::Primer3Redux::Primer

AUTHOR

cjfields <cjfields@bioperl.org>

COPYRIGHT AND LICENSE

This software is copyright (c) 2011 by Chris Fields.

This is free software; you can redistribute it and/or modify it under the same terms as the Perl 5 programming language system itself.