# BioPerl module for Bio::Factory::EMBOSS # # Please direct questions and support issues to # # Cared for by Heikki Lehvaslaiho # # Copyright Heikki Lehvaslaiho # # You may distribute this module under the same terms as perl itself # POD documentation - main docs before the code =head1 NAME Bio::Factory::EMBOSS - EMBOSS application factory class =head1 SYNOPSIS # Get an EMBOSS factory use Bio::Factory::EMBOSS; $f = Bio::Factory::EMBOSS -> new(); # Get an EMBOSS application object from the factory $water = $f->program('water') || die "Program not found!\n"; # Here is an example of running the application - water can # compare 1 sequence against 1 or more sequences using Smith-Waterman. # Pass a Sequence object and a reference to an array of objects. my $wateroutfile = 'out.water'; $water->run({-asequence => $seq_object, -bsequence => \@seq_objects, -gapopen => '10.0', -gapextend => '0.5', -outfile => $wateroutfile}); # Now you might want to get the alignment use Bio::AlignIO; my $alnin = Bio::AlignIO->new(-format => 'emboss', -file => $wateroutfile); while ( my $aln = $alnin->next_aln ) { # process the alignment -- these will be Bio::SimpleAlign objects } =head1 DESCRIPTION The EMBOSS factory class encapsulates access to EMBOSS programs. A factory object allows creation of only known applications. If you want to check command line options before sending them to the program set $prog-Everbose to positive integer. The value is passed on to programs objects and the ADC description of the available command line options is parsed and compared to input. See also L and L. =head1 FEEDBACK =head2 Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists =head2 Support Please direct usage questions or support issues to the mailing list: I rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. =head2 Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://redmine.open-bio.org/projects/bioperl/ =head1 AUTHOR - Heikki Lehvaslaiho Email heikki-at-bioperl-dot-org =head1 APPENDIX The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ =cut # Let the code begin... package Bio::Factory::EMBOSS; use vars qw(@ISA $EMBOSSVERSION); use strict; use Bio::Root::Root; use Bio::Tools::Run::EMBOSSApplication; use Bio::Factory::ApplicationFactoryI; @ISA = qw(Bio::Root::Root Bio::Factory::ApplicationFactoryI ); $EMBOSSVERSION = "2.0.0"; sub new { my($class,@args) = @_; my $self = $class->SUPER::new(@args); # set up defaults my($location) = $self->_rearrange([qw(LOCATION )], @args); $self->{ '_programs' } = {}; $self->{ '_programgroup' } = {}; $self->{ '_groups' } = {}; $self->location($location) if $location; $self->_program_list; # retrieve info about available programs return $self; } =head2 location Title : location Usage : $embossfactory->location Function: get/set the location of EMBOSS programs. Valid values are 'local' and 'novella'. Returns : string, defaults to 'local' Args : string =cut sub location { my ($self, $value) = @_; my %location = ('local' => '1', 'novella' => '1' ); if (defined $value) { $value = lc $value; if ($location{$value}) { $self->{'_location'} = $value; } else { $self->warn("Value [$value] not a valid value for ". "location(). Defaulting to [local]"); $self->{'_location'} = 'local'; } } $self->{'_location'} ||= 'local'; return $self->{'_location'}; } =head2 program Title : program Usage : $embossfactory->program('program_name') Function: Creates a representation of a single EMBOSS program and issues a warning if the program was not found. Returns : Bio::Tools::Run::EMBOSSApplication object or undef Args : string, program name =cut sub program { my ($self, $value) = @_; unless( $self->{'_programs'}->{$value} ) { $self->warn("Application [$value] is not available!"); return undef; } my $attr = {}; $attr->{name} = $value; $attr->{verbose} = $self->verbose; my $appl = Bio::Tools::Run::EMBOSSApplication->new($attr); return $appl; } =head2 version Title : $self->version Usage : $embossfactory->version() Function: gets the version of EMBOSS programs Throws : if EMBOSS suite is not accessible Returns : version value Args : None =cut sub version { my ($self) = @_; my $version = `embossversion -auto`; $self->throw("EMBOSS suite of programs is not available") if $?; chop $version; # compare versions $self->throw("EMBOSS has to be at least version $EMBOSSVERSION got $version\n") if $version lt $EMBOSSVERSION; return $version; } =head2 Programs These methods allow the programmer to query the EMBOSS suite and find out which program names can be used and what arguments can be used. =head2 program_info Title : program_info Usage : $embossfactory->program_info('emma') Function: Finds out if the program is available. Returns : definition string of the program, undef if program name not known Args : string, prgramname =cut sub program_info { my ($self, $value) = @_; return $self->{'_programs'}->{$value}; } =head2 Internal methods Do not call these methods directly =head2 _program_list Title : _program_list Usage : $embossfactory->_program_list() Function: Finds out what programs are available. Writes the names into an internal hash. Returns : true if successful Args : None =cut sub _program_list { my ($self) = @_; if( $^O =~ /Mac/i ) { return; } { my $null = ($^O =~ m/mswin/i) ? 'NUL' : '/dev/null'; local * SAVERR; open SAVERR, ">&STDERR"; open STDERR, ">$null"; open(WOSSOUT, "wossname -auto |") || return; open STDERR, ">&SAVERR"; } local $/ = "\n\n"; while( ) { my ($groupname) = (/^([A-Z][A-Z0-9 ]+)$/m); #print $groupname, "\n" if $groupname; $self->{'_groups'}->{$groupname} = [] if $groupname; while ( /^([a-z]\w+) +(.+)$/mg ) { #print "$1\t$2 \n" if $1; $self->{'_programs'}->{$1} = $2 if $1; $self->{'_programgroup'}->{$1} = $groupname if $1; push @{$self->{'_groups'}->{$groupname}}, $1 if $1; } } close(WOSSOUT); } 1;