———————————#
# BioPerl module for Bio::Tools::Run::EMBOSSacd
#
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Heikki Lehvaslaiho <heikki-at-bioperl-dot-org>
#
# Copyright Heikki Lehvaslaiho
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::EMBOSSacd - class for EMBOSS Application qualifiers
=head1 SYNOPSIS
# Get an EMBOSS factory
use Bio::Factory::EMBOSS;
$f = Bio::Factory::EMBOSS -> new();
# Get an EMBOSS application object from the factory
$water = $f->program('water') || die "Program not found!\n";
# Here is an example of running the application - water can
# compare 1 sequence against 1 or more sequences using Smith-Waterman.
# Pass a Sequence object and a reference to an array of objects.
my $wateroutfile = 'out.water';
$water->run({-asequence => $seq_object,
-bsequence => \@seq_objects,
-gapopen => '10.0',
-gapextend => '0.5',
-outfile => $wateroutfile});
# Now you might want to get the alignment
use Bio::AlignIO;
my $alnin = Bio::AlignIO->new(-format => 'emboss',
-file => $wateroutfile);
while ( my $aln = $alnin->next_aln ) {
# process the alignment -- these will be Bio::SimpleAlign objects
}
=head1 DESCRIPTION
The EMBOSSacd represents all the possible command line arguments that
can be given to an EMBOSS application.
Do not create this object directly. It will be created and attached to
its corresponding Bio::Tools::Run::EMBOSSApplication if you set
$application->verbose > 0
Call
$application->acd
to retrive the Bio::Tools::Run::EMBOSSApplication::EMBOSSacd object.
See also L<Bio::Tools::Run::EMBOSSApplication> and L<Bio::Factory::EMBOSS>.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to the
Bioperl mailing lists Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
=head1 AUTHOR - Heikki Lehvaslaiho
Email: heikki-at-bioperl-dot-org
Address:
EMBL Outstation, European Bioinformatics Institute
Wellcome Trust Genome Campus, Hinxton
Cambs. CB10 1SD, United Kingdom
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package
Bio::Tools::Run::EMBOSSacd;
use
strict;
use
Data::Dumper;
use
Bio::Root::Root;
@ISA
=
qw(Bio::Root::Root)
;
BEGIN {
%QUALIFIER_CATEGORIES
=
(
'Mandatory qualifiers'
=>
'mandatory'
,
'Standard (Mandatory) qualifiers'
=>
'mandatory'
,
'Optional qualifiers'
=>
'optional'
,
'Additional (Optional) qualifiers'
=>
'optional'
,
'Advanced qualifiers'
=>
'advanced'
,
'Advanced (Unprompted) qualifiers'
=>
'advanced'
,
'Associated qualifiers'
=>
'associated'
,
'General qualifiers'
=>
'general'
,
);
$QUAL
;
# qualifier category
}
=head2 new
Title : new
Usage : $emboss_prog->acd($prog_name);
Function: Constructor for the class.
Calls EMBOSS program 'acdc', converts the
HTML output into XML and uses XML::Twig XML
parser to write out a hash of qualifiers which is
then blessed.
Throws : without program name
Returns : new object
Args : EMBOSS program name
=cut
sub
new {
my
(
$class
,
$prog
) =
@_
;
Bio::Root::Root->
warn
(
"You need XML::Twig for EMBOSS ACD parsing"
)
and
return
undef
if
$@;
Bio::Root::Root->throw(
"Need EMBOSSprogram name as an argument"
)
unless
$prog
;
# reset global hash
%OPT
= ();
my
$version
= `embossversion -auto`;
my
$file
;
if
(
$version
lt
"2.8.0"
) {
# reading from EMBOSS program acdc stdout (prior to version 2.8.0)
$file
= `acdc
$prog
-help -verbose -acdtable 2>&1`;
}
else
{
# reading from EMBOSS program acdtable stdout (version 2.8.0 or greater)
$file
= `acdtable
$prog
-help -verbose 2>&1`;
}
# converting HTML -> XHTML for XML parsing
$file
=~ s/border/border=
"1"
/;
$file
=~ s/=(\d+)/=
"$1"
/g;
$file
=~ s/<br>/<br><\/br>/g;
$file
=~ s/
 
;//g;
my
$t
= XML::Twig->new(
TwigHandlers
=>
{
'/table/tr'
=> \
&_row
}
);
$t
->safe_parse(
$file
);
#Bio::Root::Root->throw("XML parsing error: $@");
my
%acd
=
%OPT
;
# copy to a private hash
$acd
{
'_name'
} =
$prog
;
bless
\
%acd
,
$class
;
}
sub
_row {
my
(
$t
,
$row
)=
@_
;
return
if
$row
->text eq
"(none)"
;
# no qualifiers in this category
my
$name
=
$row
->first_child;
# qualifier name
my
$namet
=
$name
->text;
if
(
$namet
=~ /qualifiers$/) {
# set category
$QUAL
=
$QUALIFIER_CATEGORIES
{
$namet
};
if
( !
defined
$QUAL
) {
warn
(
"-- namet is $namet\n"
);
}
return
;
}
my
$unnamed
= 0;
if
(
$namet
=~ /\(Parameter (\d+)\)/) {
# unnamed parameter
$unnamed
= $1;
$namet
=~ s/\(Parameter (\d+)\)//;
$namet
=~ s/[\[\]]//g ;
# name is in brackets
}
my
$desc
=
$name
->next_sibling;
my
$values
=
$desc
->next_sibling;
my
$default
=
$values
->next_sibling;
$OPT
{
$namet
}{
'unnamed'
} =
$unnamed
;
$OPT
{
$namet
}{
'category'
} =
$QUAL
;
$OPT
{
$namet
}{
'descr'
} =
$desc
->text;
$OPT
{
$namet
}{
'values'
} =
$values
->text;
$OPT
{
$namet
}{
'default'
} =
$default
->text;
$t
->purge;
# to reduce memory requirements
}
=head2 name
Title : name
Usage : $embossacd->name
Function: sets/gets the name of the EMBOSS program
Setting is done by the EMBOSSApplication object,
you should only get it.
Throws :
Returns : name string
Args : None
=cut
sub
name {
my
(
$self
,
$value
) =
@_
;
if
(
defined
$value
) {
$self
->{
'_name'
} =
$value
;
}
return
$self
->{
'_name'
};
}
=head2 print
Title : print
Usage : $embossacd->print; $embossacd->print('-word');
Function: Print out the qualifiers.
Uses Data::Dumper to print the qualifiers into STDOUT.
A valid qualifier name given as an argment limits the output.
Throws :
Returns : print string
Args : optional qualifier name
=cut
sub
{
my
(
$self
,
$value
) =
@_
;
if
(
$value
and
$self
->{
$value
}) {
Dumper
$self
->{
$value
};
}
else
{
Dumper
$self
;
}
}
=head2 mandatory
Title : mandatory
Usage : $acd->mandatory
Function: gets a mandatory subset of qualifiers
Throws :
Returns : Bio::Tools::Run::EMBOSSacd object
Args : none
=cut
sub
mandatory {
my
(
$self
) =
@_
;
my
%mand
;
foreach
my
$key
(
keys
%{
$self
}) {
next
unless
$key
=~ /^-/;
#ignore other attributes
$mand
{
$key
} =
$self
->{
$key
}
if
$self
->{
$key
}{category} eq
'mandatory'
;
}
bless
\
%mand
;
}
=head2 Qualifier queries
These methods can be used test qualifier names and read values.
=cut
=head2 qualifier
Title : qualifier
Usage : $acd->qualifier($string)
Function: tests for the existence of the qualifier
Throws :
Returns : boolean
Args : string, name of the qualifier
=cut
sub
qualifier {
my
(
$self
,
$value
) =
@_
;
$self
->throw(
"Qualifier has to start with '-'"
)
unless
$value
=~ /^-/;
$self
->{
$value
} ? 1 : 0
}
=head2 category
Title : category
Usage : $acd->category($qual_name)
Function: Return the category of the qualifier
Throws :
Returns : 'mandatory' or 'optional' or 'advanced' or
'associated' or 'general'
Args : string, name of the qualifier
=cut
sub
category {
my
(
$self
,
$value
) =
@_
;
$self
->throw(
"Not a valid qualifier name [$value]"
)
unless
$self
->qualifier(
$value
);
$self
->{
$value
}->{
'category'
};
}
=head2 values
Title : values
Usage : $acd->values($qual_name)
Function: Return the possible values for the qualifier
Throws :
Returns : string
Args : string, name of the qualifier
=cut
sub
values
{
my
(
$self
,
$value
) =
@_
;
$self
->throw(
"Not a valid qualifier name [$value]"
)
unless
$self
->qualifier(
$value
);
$self
->{
$value
}->{
'values'
};
}
=head2 descr
Title : descr
Usage : $acd->descr($qual_name)
Function: Return the description of the qualifier
Throws :
Returns : boolean
Args : string, name of the qualifier
=cut
sub
descr {
my
(
$self
,
$value
) =
@_
;
$self
->throw(
"Not a valid qualifier name [$value]"
)
unless
$self
->qualifier(
$value
);
$self
->{
$value
}->{
'descr'
};
}
=head2 unnamed
Title : unnamed
Usage : $acd->unnamed($qual_name)
Function: Find if the qualifier can be left unnamed
Throws :
Returns : 0 if needs to be named, order number otherwise
Args : string, name of the qualifier
=cut
sub
unnamed {
my
(
$self
,
$value
) =
@_
;
$self
->throw(
"Not a valid qualifier name [$value]"
)
unless
$self
->qualifier(
$value
);
$self
->{
$value
}->{
'unnamed'
};
}
=head2 default
Title : default
Usage : $acd->default($qual_name)
Function: Return the default value for the qualifier
Throws :
Returns : scalar
Args : string, name of the qualifier
=cut
sub
default
{
my
(
$self
,
$value
) =
@_
;
$self
->throw(
"Not a valid qualifier name [$value]"
)
unless
$self
->qualifier(
$value
);
$self
->{
$value
}->{
'default'
};
}
1;