NAME
Bio::DB::Query::GenBank - Build a GenBank Entrez Query
SYNOPSIS
use Bio::DB::Query::GenBank;
use Bio::DB::GenBank;
my $query_string = 'Oryza[Organism] AND EST[Keyword]';
my $query = Bio::DB::Query::GenBank->new(-db => 'nucleotide',
-query => $query_string,
-mindate => '2001',
-maxdate => '2002');
print $query->count,"\n";
# get a Genbank database handle
my $gb = Bio::DB::GenBank->new();
my $stream = $gb->get_Stream_by_query($query);
while (my $seq = $stream->next_seq) {
# do something with the sequence object
}
# initialize the list yourself
my $query = Bio::DB::Query::GenBank->new(-ids=>[195052,2981014,11127914]);DESCRIPTION
This class encapsulates NCBI Entrez queries. It can be used to store a list of GI numbers, to translate an Entrez query expression into a list of GI numbers, or to count the number of terms that would be returned by a query. Once created, the query object can be passed to a Bio::DB::GenBank object in order to retrieve the entries corresponding to the query.
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing listsSupport
Please direct usage questions or support issues to the mailing list:
bioperl-l@bioperl.org
rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible.
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
https://github.com/bioperl/bioperl-live/issuesAUTHOR - Lincoln Stein
Email lstein@cshl.org
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
new
Title : new
Usage : $db = Bio::DB::Query::GenBank->new(@args)
Function: create new query object
Returns : new query object
Args : -db database (see below for allowable values)
-query query string
-mindate minimum date to retrieve from (YYYY/MM/DD)
-maxdate maximum date to retrieve from (YYYY/MM/DD)
-reldate relative date to retrieve from (days)
-datetype date field to use ('edat' or 'mdat')
-ids array ref of gids (overrides query)
-maxids the maximum number of IDs you wish to collect
(defaults to 100)
-email Email address; required if you want to decrease
delay time between queries
-delay Delay time (in seconds). Note NCBI policy requires 4
seconds between requests unless an email is providedThis method creates a new query object. Typically you will specify a -db and a -query argument, possibly modified by -mindate, -maxdate, or -reldate. -mindate and -maxdate specify minimum and maximum dates for entries you are interested in retrieving, expressed in the form YYYY/MM/DD. -reldate is used to fetch entries that are more recent than the indicated number of days.
If you provide an array reference of IDs in -ids, the query will be ignored and the list of IDs will be used when the query is passed to a Bio::DB::GenBank object's get_Stream_by_query() method. A variety of IDs are automatically recognized, including GI numbers, Accession numbers, Accession.version numbers and locus names.
By default, the query will collect only the first 100 IDs and will generate an exception if you call the ids() method and the query returned more than that number. To increase this maximum, set -maxids to a number larger than the number of IDs you expect to obtain. This only affects the list of IDs you obtain when you call the ids() method, and does not affect in any way the number of entries you receive when you generate a SeqIO stream from the query.
-db option values:
The most commonly used databases are:
protein
nucleotide
nuccore
nucgss
nucest
unigene
An up to date list of database names supported by NCBI eUtils is
always available at:
https://eutils.ncbi.nlm.nih.gov/entrez/eutils/einfo.fcgi?
However, note that not all of these databases return datatypes that
are parsable by Bio::DB::GenBankcookie
Title : cookie
Usage : ($cookie,$querynum) = $db->cookie
Function: return the NCBI query cookie
Returns : list of (cookie,querynum)
Args : noneNOTE: this information is used by Bio::DB::GenBank in conjunction with efetch.
_request_parameters
Title : _request_parameters
Usage : ($method,$base,@params = $db->_request_parameters
Function: return information needed to construct the request
Returns : list of method, url base and key=>value pairs
Args : nonecount
Title : count
Usage : $count = $db->count;
Function: return count of number of entries retrieved by query
Returns : integer
Args : noneReturns the number of entries that are matched by the query.
ids
Title : ids
Usage : @ids = $db->ids([@ids])
Function: get/set matching ids
Returns : array of sequence ids
Args : (optional) array ref with new set of idsquery
Title : query
Usage : $query = $db->query([$query])
Function: get/set query string
Returns : string
Args : (optional) new query string_parse_response
Title : _parse_response
Usage : $db->_parse_response($content)
Function: parse out response
Returns : empty
Args : none
Throws : 'unparseable output exception'_generate_id_string
Title : _generate_id_string
Usage : $string = $db->_generate_id_string
Function: joins IDs together in string (possibly implementation-dependent)
Returns : string of concatenated IDs
Args : array ref of ids (normally passed into the constructor)delay_policy
Title : delay_policy
Usage : $secs = $self->delay_policy
Function: NCBI requests a delay of 4 seconds between requests unless email is
provided. This method implements a 4 second delay; use 'delay()' to
override, though understand if no email is provided we are not
responsible for users being IP-blocked by NCBI
Returns : number of seconds to delay
Args : none