NAME
CracTools::SAMReader - An easy to use tool to read files in SAM format.
VERSION
version 1.251
SYNOPSIS
use CracTools::SAMReader;
# Creating the reader
my $sam_reader = CracTools::SAMreader->new($sam,'CRAC');
# Get an iterator to go through the SAM file in a linear way
my $it = $sam_reader->iterator();
# Iterate on lines and explore CRAC special fields of SAM
while(my $line = $it->()) {
if(defined $line->events('Junction') && $line->isClassified('normal')) {
my @junctions = @{$line->events('Junction')};
foreach my $junction (@junctions) {
print "Foud Junction : [type : $junction->{type}, loc : $junction->{loc}, gap : $junction->{gap}]\n";
}
}
}
DESCRIPTION
Reader for SAM format, including CRAC special fields.
METHODS
new
Arg [1] : String - SAM file
Arg [2] : (Optional) String - SAM type
- CRAC
- CRAC_EMT
Example : $reader = CracTools::SAMreader->new('file.sam','CRAC');
Description : Create a new reader object
ReturnType : CracTools::SAMreader
Exceptions : none
iterator
Example : my $it = $sam_reader->iterator();
while(my $line = $it->()) {
print $line->seq,"\n";
}
Description : Create an iterator to go throud each lines of the file
ReturnType : Iterator on CracTools::SAMline
Exceptions : none
iteratorFile
Arg [1] : (Optional) String - options (IGNORE_HEADERS,..)
Example : my $it_f = $sam_reader->iteratorFile();
while(my ($line,$line_number) = $it->()) {
print $line,"\n";
}
Description : Create an iterator to go throud each lines of the file
ReturnType : Iterator on Array (String,Int) where the <String> is the
line, and <Int> the line number.
Exceptions : none
GETTERS AND SETTERS
header
Description : Getter/setter for attribute header
ReturnType : none
Exceptions : none
refSeqLength
Description : Return the length of the reference sequence given in argument
ReturnType : Integer
allRefSeqLengths
Return a hashref with all reference sequence names and length.
my $refseq_lengths = $sam_reader->allRefSeqLength();
The return value is :
{ refseq_name => length,
refseq_name => lenght,
...
}
commandLine
Description : Return crac command line defined in SAM's header
ReturnType : String
getCracArgumentValue
Description : Retrun the value of the specified argument in crac command line
hasCracOption
Description : Return true if crac command line has specified a certain option
getCracVersionNumber
Description : Return CRAC version number
PRIVATE METHODS
init (private)
Description : Initialization method
ReturnType : none
Exceptions : none
AUTHORS
Nicolas PHILIPPE <nphilippe.research@gmail.com>
Jérôme AUDOUX <jaudoux@cpan.org>
Sacha BEAUMEUNIER <sacha.beaumeunier@gmail.com>
COPYRIGHT AND LICENSE
This software is Copyright (c) 2017 by IRMB/INSERM (Institute for Regenerative Medecine and Biotherapy / Institut National de la Santé et de la Recherche Médicale) and AxLR/SATT (Lanquedoc Roussilon / Societe d'Acceleration de Transfert de Technologie).
This is free software, licensed under:
The GNU Affero General Public License, Version 3, November 2007