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LICENSE

Copyright [1999-2015] Wellcome Trust Sanger Institute and the EMBL-European Bioinformatics Institute Copyright [2016-2024] EMBL-European Bioinformatics Institute

Licensed under the Apache License, Version 2.0 (the "License"); you may not use this file except in compliance with the License. You may obtain a copy of the License at

     http://www.apache.org/licenses/LICENSE-2.0

Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.

CONTACT

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  Questions may also be sent to the Ensembl help desk at
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NAME

Bio::EnsEMBL::Utils::PolyA

SYNOPSIS

  my $seq;    # a Bio::Seq object
  my $polyA = Bio::EnsEMBL::Utils::PolyA->new();

  # returns a new Bio::Seq object with the trimmed sequence
  my $trimmed_seq = $polyA->clip($seq);

  # cat put Ns in the place of the polyA/polyT tail
  my $masked_seq = $polyA->mask($seq);

  # can put in lower case the polyA/polyT using any flag:
  my $softmasked_seq = $poly->mask( $seq, 'soft' );

DESCRIPTION

  It reads a Bio::Seq object, it first finds out whether it has a
  polyA or a polyT and then performs one operation in the seq string:
  clipping, masking or softmasking.  It then returns a new Bio::Seq
  object with the new sequence.

METHODS

new