NAME

Bio::AssemblyImprovement::DigitalNormalisation::Khmer::Main - Perform digital normalisation on sequencing reads

VERSION

version 1.160490

SYNOPSIS

Runs digital normalisation using khmer. Final results file (zipped) produced in directory of your choice (default: current working directory)

my $digi_norm = Bio::AssemblyImprovement::DigitalNormalisation::Khmer::Main->new( input_file => 'abc_shuffled.fastq', desired_coverage => '40', khmer_exec => '/path/to/normalise/script.py', python_exec => 'python-2.7', )->run; my $results = $digi_norm->_final_results_file;

METHODS

run

_final_results_file

Return full path to the results file

AUTHOR

Andrew J. Page <ap13@sanger.ac.uk>

COPYRIGHT AND LICENSE

This software is Copyright (c) 2012 by Wellcome Trust Sanger Institute.

This is free software, licensed under:

The GNU General Public License, Version 3, June 2007