NAME
Bio::AlignIO::phylip - PHYLIP format sequence input/output stream
SYNOPSIS
# Do not use this module directly. Use it via the Bio::AlignIO class.
use Bio::AlignIO;
use Bio::SimpleAlign;
#you can set the name length to something other than the default 10
#if you use a version of phylip (hacked) that accepts ids > 10
my $phylipstream = new Bio::AlignIO(-format => 'phylip',
-fh => \*STDOUT,
-idlength=>30);
# convert data from one format to another
my $gcgstream = new Bio::AlignIO(-format => 'msf',
-file => 't/data/cysprot1a.msf');
while( my $aln = $gcgstream->next_aln ) {
$phylipstream->write_aln($aln);
}
# do it again with phylip sequential format format
$phylipstream->interleaved(0);
# can also initialize the object like this
$phylipstream = new Bio::AlignIO(-interleaved => 0,
-format => 'phylip',
-fh => \*STDOUT,
-idlength=>10);
$gcgstream = new Bio::AlignIO(-format => 'msf',
-file => 't/data/cysprot1a.msf');
while( my $aln = $gcgstream->next_aln ) {
$phylipstream->write_aln($aln);
}
DESCRIPTION
This object can transform Bio::SimpleAlign objects to and from PHYLIP interleaved format. It will not work with PHYLIP sequencial format.
This module will output PHYLIP sequential format. By specifying the flag -interleaved => 0 in the initialization the module can output data in interleaved format.
FEEDBACK
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web:
bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/
AUTHORS - Heikki Lehvaslaiho and Jason Stajich
Email: heikki@ebi.ac.uk Email: jason@bioperl.org
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
new
Title : new
Usage : my $alignio = new Bio::AlignIO(-format => 'phylip'
-file => '>file',
-idlength => 10,
-idlinebreak => 1);
Function: Initialize a new L<Bio::AlignIO::phylip> reader or writer
Returns : L<Bio::AlignIO> object
Args : [specific for writing of phylip format files]
-idlength => integer - length of the id (will pad w/
spaces if needed)
-interleaved => boolean - whether or not write as interleaved
or sequential format
-linelength => integer of how long a sequence lines should be
-idlinebreak => insert a line break after the sequence id
so that sequence starts on the next line
next_aln
Title : next_aln
Usage : $aln = $stream->next_aln()
Function: returns the next alignment in the stream.
Throws an exception if trying to read in PHYLIP
sequential format.
Returns : L<Bio::SimpleAlign> object
Args :
write_aln
Title : write_aln
Usage : $stream->write_aln(@aln)
Function: writes the $aln object into the stream in MSF format
Returns : 1 for success and 0 for error
Args : L<Bio::Align::AlignI> object
interleaved
Title : interleaved
Usage : my $interleaved = $obj->interleaved
Function: Get/Set Interleaved status
Returns : boolean
Args : boolean
idlength
Title : idlength
Usage : my $idlength = $obj->interleaved
Function: Get/Set value of id length
Returns : string
Args : string
line_length
Title : line_length
Usage : $obj->line_length($newval)
Function:
Returns : value of line_length
Args : newvalue (optional)
id_linebreak
Title : id_linebreak
Usage : $obj->id_linebreak($newval)
Function:
Returns : value of id_linebreak
Args : newvalue (optional)