/ 
bioperl-1.4
/ Bio::Variation::DNAMutation

NAME

Bio::Variation::DNAMutation - DNA level mutation class

SYNOPSIS

    $dnamut = Bio::Variation::DNAMutation->new
        ('-start'         => $start,
         '-end'           => $end,
         '-length'        => $len,
         '-upStreamSeq'   => $upflank,
         '-dnStreamSeq'   => $dnflank,
         '-proof'         => $proof,
	 '-isMutation'    => 1,
         '-mut_number'    => $mut_number
        );
    $a1 = Bio::Variation::Allele->new;
    $a1->seq('a');
    $dnamut->allele_ori($a1);
    my $a2 = Bio::Variation::Allele->new;
    $a2->seq('t');
    $dnamut->add_Allele($a2);

    print "Restriction changes are ", $dnamut->restriction_changes, "\n";

    # add it to a SeqDiff container object
    $seqdiff->add_Variant($dnamut);

DESCRIPTION

The instantiable class Bio::Variation::DNAMutation describes basic sequence changes in genomic DNA level. It uses methods defined in superclass Bio::Variation::VariantI. See Bio::Variation::VariantI for details.

If the variation described by a DNAMutation object is transcibed, link the corresponding Bio::Variation::RNAChange object to it using method RNAChange(). See Bio::Variation::RNAChange for more information.

FEEDBACK

Mailing Lists

User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing lists Your participation is much appreciated.

bioperl-l@bioperl.org                         - General discussion
http://bio.perl.org/MailList.html             - About the mailing lists

Reporting Bugs

report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via email or the web:

bioperl-bugs@bio.perl.org
http://bugzilla.bioperl.org/

AUTHOR - Heikki Lehvaslaiho

Email: heikki@ebi.ac.uk Address:

EMBL Outstation, European Bioinformatics Institute
Wellcome Trust Genome Campus, Hinxton
Cambs. CB10 1SD, United Kingdom

APPENDIX

The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _

CpG

Title   : CpG
Usage   : $obj->CpG()
Function: sets and returns boolean values for variation 
          hitting a CpG site.  Unset value return -1.
Example : $obj->CpG()
Returns : boolean
Args    : optional true of false value

RNAChange

Title   : RNAChange
Usage   : $mutobj = $obj->RNAChange;
        : $mutobj = $obj->RNAChange($objref);
Function: Returns or sets the link-reference to a mutation/change object.
          If there is no link, it will return undef
Returns : an obj_ref or undef

label

Title   : label
Usage   : $obj->label();
Function: 

           Sets and returns mutation event label(s).  If value is not
           set, or no argument is given returns false.  Each
           instantiable subclass of L<Bio::Variation::VariantI> needs
           to implement this method. Valid values are listed in
           'Mutation event controlled vocabulary' in
           http://www.ebi.ac.uk/mutations/recommendations/mutevent.html.

Example : 
Returns : string
Args    : string

sysname

Title   : sysname
Usage   : $self->sysname
Function: 

          This subroutine creates a string corresponding to the
          'systematic name' of the mutation. Systematic name is
          specified in Antonorakis & MDI Nomenclature Working Group:
          Human Mutation 11:1-3, 1998. 
          http://www.interscience.wiley.com/jpages/1059-7794/nomenclature.html
Returns : string