——————#
# BioPerl module for Bio::SeqIO::MultiFile
#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by Ewan Birney <birney@ebi.ac.uk>
#
# Copyright Ewan Birney
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SeqIO::MultiFile - Treating a set of files as a single input stream
=head1 SYNOPSIS
my $seqin = Bio::SeqIO::MultiFile->new( -format => 'Fasta',
-files => ['file1','file2'] );
while (my $seq = $seqin->next_seq) {
# do something with $seq
}
=head1 DESCRIPTION
Bio::SeqIO::MultiFile provides a simple way of bundling a whole
set of identically formatted sequence input files as a single stream.
File format is automatically determined by C<Bio::SeqIO>.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via the web:
=head1 AUTHOR - Ewan Birney
Email birney@ebi.ac.uk
=head1 APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
=cut
# Let the code begin...
package
Bio::SeqIO::MultiFile;
$Bio::SeqIO::MultiFile::VERSION
=
'1.7.8'
;
use
strict;
# _initialize is where the heavy stuff will happen when new is called
sub
_initialize {
my
(
$self
,
@args
) =
@_
;
$self
->SUPER::_initialize(
@args
);
my
(
$file_array
,
$format
) =
$self
->_rearrange([
qw(FILES FORMAT)
],
@args
);
if
( !
defined
$file_array
|| !
ref
$file_array
) {
$self
->throw(
"Must have an array files for MultiFile"
);
}
$self
->{
'_file_array'
} = [];
$self
->_set_file(
@$file_array
);
$self
->
format
(
$format
)
if
defined
$format
;
if
(
$self
->_load_file() == 0 ) {
$self
->throw(
"Unable to initialise the first file"
);
}
}
=head2 next_seq
Title : next_seq
Usage :
Function:
Example :
Returns :
Args :
=cut
sub
next_seq{
my
(
$self
,
@args
) =
@_
;
my
$seq
=
$self
->_current_seqio->next_seq();
if
( !
defined
$seq
) {
if
(
$self
->_load_file() == 0) {
return
;
}
else
{
return
$self
->next_seq();
}
}
else
{
return
$seq
;
}
}
=head2 next_primary_seq
Title : next_primary_seq
Usage :
Function:
Example :
Returns :
Args :
=cut
sub
next_primary_seq{
my
(
$self
,
@args
) =
@_
;
my
$seq
=
$self
->_current_seqio->next_primary_seq();
if
( !
defined
$seq
) {
if
(
$self
->_load_file() == 0) {
return
;
}
else
{
return
$self
->next_primary_seq();
}
}
else
{
return
$seq
;
}
}
=head2 _load_file
Title : _load_file
Usage :
Function:
Example :
Returns :
Args :
=cut
sub
_load_file{
my
(
$self
,
@args
) =
@_
;
my
$file
=
shift
@{
$self
->{
'_file_array'
}};
if
( !
defined
$file
) {
return
0;
}
my
$seqio
;
my
$format
=
$self
->
format
;
if
(
$format
) {
$seqio
= Bio::SeqIO->new(
-file
=>
$file
,
-format
=>
$format
);
}
else
{
$seqio
= Bio::SeqIO->new(
-file
=>
$file
);
$self
->
format
(
$seqio
->
format
)
if
not
$format
;
}
# should throw an exception - but if not...
if
( !
defined
$seqio
) {
$self
->throw(
"Could not build SeqIO object for $file!"
);
}
$self
->_current_seqio(
$seqio
);
return
1;
}
=head2 _set_file
Title : _set_file
Usage :
Function:
Example :
Returns :
Args :
=cut
sub
_set_file{
my
(
$self
,
@files
) =
@_
;
push
@{
$self
->{
'_file_array'
}},
@files
;
}
=head2 _current_seqio
Title : _current_seqio
Usage : $obj->_current_seqio($newval)
Function:
Example :
Returns : value of _current_seqio
Args : newvalue (optional)
=cut
sub
_current_seqio{
my
(
$obj
,
$value
) =
@_
;
if
(
defined
$value
) {
$obj
->{
'_current_seqio'
} =
$value
;
}
return
$obj
->{
'_current_seqio'
};
}
# We overload the format() method of Bio::Root::IO by a simple get/set
sub
format
{
my
(
$obj
,
$value
) =
@_
;
if
(
defined
$value
) {
$obj
->{
'_format'
} =
$value
;
}
return
$obj
->{
'_format'
};
}
1;