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#-----------------------------------------------------------------------------
# PACKAGE : Bio::SeqIO::raw
# AUTHOR : Ewan Birney <birney@ebi.ac.uk>
# CREATED : Feb 16 1999
#
# Copyright (c) 1997-9 bioperl, Ewan Birney. All Rights Reserved.
# This module is free software; you can redistribute it and/or
# modify it under the same terms as Perl itself.
#
# _History_
#
# Ewan Birney <birney@ebi.ac.uk> developed the SeqIO
# schema and the first prototype modules.
#
# This code is based on his Bio::SeqIO::Fasta module with
# the necessary minor tweaks necessary to get it to read
# and write raw formatted sequences made by
# chris dagdigian <dag@sonsorol.org>
#
# October 18, 1999 Largely rewritten by Lincoln Stein
#
# Copyright Ewan Birney
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::SeqIO::raw - raw sequence file input/output stream
=head1 SYNOPSIS
Do not use this module directly. Use it via the L<Bio::SeqIO> class.
=head1 DESCRIPTION
This object can transform Bio::Seq objects to and from raw flat
file databases.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution.
Bug reports can be submitted via the web:
=head1 AUTHORS
Ewan Birney E<lt>birney@ebi.ac.ukE<gt>
Lincoln Stein E<lt>lstein@cshl.orgE<gt>
=head1 CONTRIBUTORS
Jason Stajich E<lt>jason@bioperl.org<gt>
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
$Bio::SeqIO::raw::VERSION = '1.7.8';
use strict;
use base qw(Bio::SeqIO);
our %variant = ( 'multiple' => undef, # default
'single' => undef );
sub _initialize {
my($self,@args) = @_;
$self->SUPER::_initialize(@args);
my ($variant) = $self->_rearrange([qw(VARIANT)], @args);
$variant ||= 'multiple';
$self->variant($variant);
$self->{record_separator} = $variant eq 'single' ? undef : $/;
if( ! defined $self->sequence_factory ) {
$self->sequence_factory(Bio::Seq::SeqFactory->new
(-verbose => $self->verbose(),
-type => 'Bio::Seq'));
}
$self->variant;
}
=head2 next_seq
Title : next_seq
Usage : $seq = $stream->next_seq()
Function: returns the next sequence in the stream
Returns : Bio::Seq object
Args :
=cut
sub next_seq{
my ($self,@args) = @_;
## When its 1 sequence per line with no formatting at all,
## grabbing it should be easy :)
## adding an option to assume the file is one sequence
local $/ = $self->{record_separator};
my $nextline = $self->_readline();
return unless defined $nextline;
my $sequence = uc($nextline);
$sequence =~ s/\W//g;
return unless $sequence;
return $self->sequence_factory->create(-seq => $sequence) if $sequence;
}
=head2 write_seq
Title : write_seq
Usage : $stream->write_seq($seq)
Function: writes the $seq object into the stream
Returns : 1 for success and 0 for error
Args : Array of Bio::PrimarySeqI objects
=cut
sub write_seq {
my ($self,@seq) = @_;
foreach my $seq (@seq) {
$self->throw("Must provide a valid Bio::PrimarySeqI object")
unless defined $seq && ref($seq) && $seq->isa('Bio::PrimarySeqI');
$self->_print($seq->seq, "\n") or return;
}
$self->flush if $self->_flush_on_write && defined $self->_fh;
return 1;
}
=head2 write_qual
Title : write_qual
Usage : $stream->write_qual($seq)
Function: writes the $seq object into the stream
Returns : 1 for success and 0 for error
Args : Bio::Seq::Quality object
=cut
sub write_qual {
my ($self,@seq) = @_;
my @qual = ();
foreach (@seq) {
unless ($_->isa("Bio::Seq::Quality")){
warn("You cannot write raw qualities without supplying a Bio::Seq::".
"Quality object! You passed a ".ref($_)."\n");
next;
}
@qual = @{$_->qual};
if(scalar(@qual) == 0) {
$qual[0] = "\n";
}
$self->_print (join " ", @qual,"\n") or return;
}
return 1;
}
=head2 variant
Title : variant
Usage : $format = $obj->variant();
Function: Get and set method for the sequence variant. For raw sequence, this
indicates whether to treat the input as multiple sequences (the
default) or as a single sequence.
Current values accepted are:
'single' single sequence
'multiple' multiple sequences (default)
Returns : string
Args : new value, string
=cut
# variant() method inherited from Bio::Root::IO
# private method for testing record separator
sub _separator {
shift->{record_separator};
}
1;