From Code to Community: Sponsoring The Perl and Raku Conference 2025 Learn more

=head1 NAME
Bio::Tools::TandemRepeatsFinder - a parser for Tandem Repeats Finder output
=head1 SYNOPSIS
use Bio::Tools::TandemRepeatsFinder;
# create parser
my $parser = Bio::Tools::Bio::Tools::TandemRepeatsFinder->new(-file => 'tandem_repeats.out');
# loop through results
while( my $feature = $parser->next_result ) {
# print the source sequence id, start, end, percent matches, and the consensus sequence
my ($percent_matches) = $feat->get_tag_values('percent_matches');
my ($consensus_sequence) = $feat->get_tag_values('consensus_sequence');
print $feat->seq_id()."\t".$feat->start()."\t".$feat->end()."\t$percent_matches\t$consensus_sequence\n";
}
=head1 DESCRIPTION
A parser for Tandem Repeats Finder output.
Written and tested for version 4.00
Location, seq_id, and score are stored in Bio::SeqFeature::Generic feature.
All other data is stored in tags. The available tags are
period_size
copy_number
consensus_size
percent_matches
percent_indels
percent_a
percent_c
percent_g
percent_t
entropy
consensus_sequence
repeat_sequence
run_parameters
sequence_description
The run_parameters are stored in a hashref with the following key:
match_weight
mismatch_weight
indel_weight
match_prob
indel_prob
min_score
max_period_size
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
=head1 AUTHOR - Eric Just
Email e-just@northwestern.edu
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
$Bio::Tools::TandemRepeatsFinder::VERSION = '1.7.8';
use strict;
use constant DEBUG => 0;
use base qw(Bio::Root::Root Bio::Root::IO);
=head2 new
Title : new
Usage : my $obj = Bio::Tools::TandemRepeatsFinder->new();
Function: Builds a new Bio::Tools::TandemRepeatsFinder object
Returns : Bio::Tools::TandemRepeatsFinder
Args : -fh/-file => $val, for initing input, see Bio::Root::IO
=cut
sub new {
my ( $class, @args ) = @_;
my $self = $class->SUPER::new(@args);
$self->_initialize_io(@args);
return $self;
}
=head2 version
Title : version
Usage : $self->version( $version )
Function: get/set the version of Tandem Repeats finder that was used in analysis
Returns : value of version of
Args : new value (optional)
=cut
sub version {
my ( $self, $value ) = @_;
if ( defined $value ) {
$self->{'version'} = $value;
}
return $self->{'version'};
}
=head2 _current_seq_id
Title : _current_seq_id
Usage : $self->_current_seq_id( $current_seq_id )
Function: get/set the _current_seq_id
Returns : value of _current_seq_id
Args : new value (optional)
=cut
sub _current_seq_id {
my ( $self, $value ) = @_;
if ( defined $value ) {
$self->{'_current_seq_id'} = $value;
}
return $self->{'_current_seq_id'};
}
=head2 _current_seq_description
Title : _current_seq_description
Usage : $self->_current_seq_description( $current_seq_id )
Function: get/set the _current_seq_description
Returns : value of _current_seq_description
Args : new value (optional)
=cut
sub _current_seq_description {
my ( $self, $value ) = @_;
if ( defined $value ) {
$self->{'_current_seq_description'} = $value;
}
return $self->{'_current_seq_description'};
}
=head2 _current_parameters
Title : _current_parameters
Usage : $self->_current_parameters( $parameters_hashref )
Function: get/set the _current_parameters
Returns : hashref representing current parameters parsed from results file
: keys are
match_weight
mismatch_weight
indel_weight
match_prob
indel_prob
min_score
max_period_size
Args : parameters hashref (optional)
=cut
sub _current_parameters {
my ( $self, $value ) = @_;
if ( defined $value ) {
$self->{'_current_parameters'} = $value;
}
return $self->{'_current_parameters'};
}
=head2 next_result
Title : next_result
Usage : my $r = $trf->next_result()
Function: Get the next result set from parser data
Returns : Bio::SeqFeature::Generic
Args : none
=cut
sub next_result {
my ($self) = @_;
while ( defined( $_ = $self->_readline() ) ) {
# Parse Version line
if (/^Version (.+)/) {
my $version = $1;
$self->warn("parsed version: $version\n") if DEBUG;
$self->warn( qq{ Bio::Tools::TandemRepeatsFinder was written and tested for Tandem Repeats Masker Version 4.00 output
You appear to be using Verion $version. Use at your own risk.}) if ($version != 4);
$self->version($version);
}
# Parse Sequence identifier
# i.e. Sequence: DDB0215018 |Masked Chromosomal Sequence| Chr 2f
elsif ( /^Sequence: ([^\s]+)\s(.+)?/ ) {
my $seq_id = $1;
my $seq_description = $2;
$self->warn("parsed sequence_id: $seq_id\n") if DEBUG;
$self->_current_seq_id($seq_id);
$self->_current_seq_description($seq_description);
}
# Parse Parameters
# i.e. Parameters: 2 7 7 80 10 50 12
elsif (/^Parameters: (.+)/) {
my $params = $1;
$self->warn("parsed parameters: $params\n") if DEBUG;
my @param_array = split /\s/, $params;
my $param_hash = {
match_weight => $param_array[0],
mismatch_weight => $param_array[1],
indel_weight => $param_array[2],
match_prob => $param_array[3],
indel_prob => $param_array[4],
min_score => $param_array[5],
max_period_size => $param_array[6]
};
$self->_current_parameters($param_hash);
}
# Parse Data
# i.e. 13936 13960 12 2.1 12 100 0 50 16 8 52 24 1.70 T TTTTTTTTTT
elsif (/^\d+\s\d+\s\d+/) {
# call internal method to create Bio::SeqFeature::Generic
# to represent tandem repeat
return $self->_create_feature($_);
}
elsif (DEBUG) {
$self->warn( "UNPARSED LINE:\n" . $_ );
}
}
return;
}
=head2 _create_feature
Title : _create_feature
Usage : internal method used by 'next_feature'
Function: Takes a line from the results file and creates a bioperl object
Returns : Bio::SeqFeature::Generic
Args : none
=cut
sub _create_feature {
my ( $self, $line ) = @_;
# split the line and store into named variables
my @element = split /\s/, $line;
my (
$start, $end, $period_size,
$copy_number, $consensus_size, $percent_matches,
$percent_indels, $score, $percent_a,
$percent_c, $percent_g, $percent_t,
$entropy, $consensus_sequence, $repeat_sequence
) = @element;
# create tag hash from data in line
my $tags = {
period_size => $period_size,
copy_number => $copy_number,
consensus_size => $consensus_size,
percent_matches => $percent_matches,
percent_indels => $percent_indels,
percent_a => $percent_a,
percent_c => $percent_c,
percent_g => $percent_g,
percent_t => $percent_t,
entropy => $entropy,
consensus_sequence => $consensus_sequence,
repeat_sequence => $repeat_sequence,
run_parameters => $self->_current_parameters(),
sequence_description => $self->_current_seq_description()
};
# create feature from start/end etc
my $feat = Bio::SeqFeature::Generic->new(
-seq_id => $self->_current_seq_id(),
-score => $score,
-start => $start,
-end => $end,
-source_tag => 'Tandem Repeats Finder',
-primary_tag => 'tandem repeat',
-tag => $tags
);
return $feat;
}
1;