FASTX-Abi

• Master branch

• Test branch (if any)

OOP Perl module to convert Sanger trace files (.ab1) to FASTQ sequences. Ambiguities (hetero bases) are managed.

Homepage: FASTX::Abi (MetaCPAN)

The picture shows a trace file (chromatogram, in abi/ab1 format) with a SNP detected and the typical quality degradation at the end.