NAME
Bio::SeqIO::swiss - Swissprot sequence input/output stream
SYNOPSIS
It is probably best not to use this object directly, but rather go through the SeqIO handler system:
use Bio::SeqIO;
$stream = Bio::SeqIO->new(-file => $filename,
-format => 'swiss');
while ( my $seq = $stream->next_seq() ) {
# do something with $seq
}
DESCRIPTION
This object can transform Bio::Seq objects to and from Swissprot flat file databases.
There is a lot of flexibility here about how to dump things which needs to be documented.
Optional functions
- _show_dna()
-
(output only) shows the dna or not
- _post_sort()
-
(output only) provides a sorting func which is applied to the FTHelpers before printing
- _id_generation_func()
-
This is function which is called as
print "ID ", $func($seq), "\n";
To generate the ID line. If it is not there, it generates a sensible ID line using a number of tools.
If you want to output annotations in Swissprot format they need to be stored in a Bio::Annotation::Collection object which is accessible through the Bio::SeqI interface method annotation().
The following are the names of the keys which are polled from a Bio::Annotation::Collection object.
reference - Should contain Bio::Annotation::Reference objects comment - Should contain Bio::Annotation::Comment objects dblink - Should contain Bio::Annotation::DBLink objects gene_name - Should contain Bio::Annotation::SimpleValue object
FEEDBACK
Mailing Lists
User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions, preferably to one of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web:
http://bugzilla.open-bio.org/
AUTHOR - Elia Stupka
Email elia@tll.org.sg
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
next_seq
Title : next_seq
Usage : $seq = $stream->next_seq()
Function: returns the next sequence in the stream
Returns : Bio::Seq object
Args :
write_seq
Title : write_seq
Usage : $stream->write_seq($seq)
Function: writes the $seq object (must be seq) to the stream
Returns : 1 for success and 0 for error
Args : array of 1 to n Bio::SeqI objects
_generateCRCTable
Title : _generateCRCTable
Usage :
Function:
Example :
Returns :
Args :
_crc32
Title : _crc32
Usage :
Function:
Example :
Returns :
Args :
_crc64
Title : _crc64
Usage :
Function:
Example :
Returns :
Args :
_print_swissprot_FTHelper
Title : _print_swissprot_FTHelper
Usage :
Function:
Example :
Returns :
Args :
_read_swissprot_References
Title : _read_swissprot_References
Usage :
Function: Reads references from swissprot format. Internal function really
Example :
Returns :
Args :
_read_swissprot_Species
Title : _read_swissprot_Species
Usage :
Function: Reads the swissprot Organism species and classification
lines.
Able to deal with unconventional species names.
Example : OS Unknown prokaryotic organism
$genus = undef ; $species = Unknown prokaryotic organism
Returns : A Bio::Species object
Args :
_filehandle
Title : _filehandle
Usage : $obj->_filehandle($newval)
Function:
Example :
Returns : value of _filehandle
Args : newvalue (optional)
_read_FTHelper_swissprot
Title : _read_FTHelper_swissprot
Usage : _read_FTHelper_swissprot(\$buffer)
Function: reads the next FT key line
Example :
Returns : Bio::SeqIO::FTHelper object
Args :
_write_line_swissprot
Title : _write_line_swissprot
Usage :
Function: internal function
Example :
Returns :
Args :
_write_line_swissprot_regex
Title : _write_line_swissprot_regex
Usage :
Function: internal function for writing lines of specified
length, with different first and the next line
left hand headers and split at specific points in the
text
Example :
Returns : nothing
Args : file handle, first header, second header, text-line, regex for line breaks, total line length
_post_sort
Title : _post_sort
Usage : $obj->_post_sort($newval)
Function:
Returns : value of _post_sort
Args : newvalue (optional)
_show_dna
Title : _show_dna
Usage : $obj->_show_dna($newval)
Function:
Returns : value of _show_dna
Args : newvalue (optional)
_id_generation_func
Title : _id_generation_func
Usage : $obj->_id_generation_func($newval)
Function:
Returns : value of _id_generation_func
Args : newvalue (optional)
_ac_generation_func
Title : _ac_generation_func
Usage : $obj->_ac_generation_func($newval)
Function:
Returns : value of _ac_generation_func
Args : newvalue (optional)
_sv_generation_func
Title : _sv_generation_func
Usage : $obj->_sv_generation_func($newval)
Function:
Returns : value of _sv_generation_func
Args : newvalue (optional)
_kw_generation_func
Title : _kw_generation_func
Usage : $obj->_kw_generation_func($newval)
Function:
Returns : value of _kw_generation_func
Args : newvalue (optional)