# $Id: INSTALL,v 1.47.4.6 2006/11/21 10:33:26 sendu Exp $
Installing Bioperl for Unix
* BIOPERL INSTALLATION
* SYSTEM REQUIREMENTS
* OPTIONAL
* ADDITIONAL INSTALLATION INFORMATION
* THE BIOPERL BUNDLE
* INSTALLING BIOPERL THE EASY WAY USING CPAN
* INSTALLING BIOPERL THE EASY WAY USING Build.PL
* WHERE ARE THE MAN PAGES?
* EXTERNAL PROGRAMS
* Environment Variables
* INSTALLING BIOPERL SCRIPTS
* INSTALLING BIOPERL IN A PERSONAL MODULE AREA
* INSTALLING BIOPERL MODULES THE HARD WAY
* USING MODULES NOT INSTALLED IN THE STANDARD LOCATION
* THE TEST SYSTEM
* BUILDING THE OPTIONAL bioperl-ext PACKAGE
* CONFIGURING for BSD and Solaris boxes
* INSTALLATION
BIOPERL INSTALLATION
Bioperl has been installed on many forms of Unix,
Win9X/NT/2000/XP, and on Mac OS X (see the PLATFORMS file for more
details). Following are instructions for installing Bioperl for
Unix/Linux/Mac OS X; Windows installation instructions can be found
in INSTALL.WIN. For installing Bioperl for Mac OS X using Fink, see:
SYSTEM REQUIREMENTS
* Perl 5.6.1 or later; version 5.8 and greater are recommended.
* External modules: Bioperl uses functionality provided in other
Perl modules. Some of these are included in the standard perl package
but some need to be obtained from the CPAN site. The list of external
modules is included in the DEPENDENCIES file.
When not using CPAN to install Bioperl itself, the CPAN Bioperl Bundle
(Bundle::BioPerl) makes installation of these external modules easy. Simply
install the bundle using your CPAN shell and many optional modules will be
installed. See THE BIOPERL BUNDLE, below. However, it is recommended you use
CPAN to install Bioperl and not use Bundle::BioPerl.
OPTIONAL
* ANSI C or GNU C compiler (gcc) for XS extensions (the
bioperl-ext package; see BUILDING THE OPTIONAL bioperl-ext
PACKAGE, below).
ADDITIONAL INSTALLATION INFORMATION
* Additional information on Bioperl and MAC OS:
* OS X - Installing using Fink (in Getting BioPerl)
THE BIOPERL BUNDLE
You do not need to install Bundle::BioPerl if you will install Bioperl
using CPAN. This is for users manually installing Bioperl from archives
or CVS, who also want an easy way to install the optional external
modules that Bioperl makes use of.
Bundle::BioPerl does *not* install Bioperl.
You typically need root privileges to install using CPAN. If you don't
have these privileges please see INSTALLING BIOPERL IN A PERSONAL
MODULE AREA for additional information.
Install Bundle::Bioperl using CPAN. One way:
>perl -MCPAN -e "install Bundle::BioPerl"
Another way:
>perl -MCPAN -e shell
cpan>install Bundle::BioPerl
INSTALLING BIOPERL THE EASY WAY USING CPAN
You can use the CPAN shell to install Bioperl. For example:
>perl -MCPAN -e shell
Then find the name of the Bioperl version you want:
cpan>d /bioperl/
CPAN: Storable loaded ok
Going to read /home/bosborne/.cpan/Metadata
Database was generated on Mon, 20 Nov 2006 05:24:36 GMT
Distribution B/BI/BIRNEY/bioperl-1.2.tar.gz
Distribution B/BI/BIRNEY/bioperl-1.4.tar.gz
Distribution S/SE/SENDU/bioperl-1.5.2_100.tar.gz
Now install:
cpan>install S/SE/SENDU/bioperl-1.5.2_100.tar.gz
If you've installed everything perfectly and all the network connections
are working then you may pass all the tests run in the 'make test' phase.
It's also possible that you may fail some tests. Possible explanations:
problems with local Perl installation, network problems, previously
undetected bug in Bioperl, flawed test script, problems with CGI
script used for sequence retrieval at public database, and so on. Remember
that there are over 800 modules in Bioperl and the test suite is running
more than 12000 individual tests, a few failed tests may not affect your
usage of Bioperl.
If you decide that the failed tests will not affect how you intend to use
Bioperl and you'd like to install anyway do:
cpan>force install S/SE/SENDU/bioperl-1.5.2_100.tar.gz
This is what most experienced Bioperl users would do. However, if you're
concerned about a failed test and need assistance or advice then contact
bioperl-l@bioperl.org.
INSTALLING BIOPERL THE EASY WAY USING Build.PL
The advantage of this approach is it's stepwise, so it's easy to stop and
analyze in case of any problem.
Download, then unpack the tar file. For example:
>gunzip bioperl-1.5.2_100.tar.gz
>tar xvf bioperl-1.5.2_100.tar
>cd bioperl-1.5.2_100
Now issue the make commands:
>perl Build.PL
>./Build test
If you've installed everything perfectly and all the network connections
are working then you may pass all the tests run in the 'make test' phase.
It's also possible that you may fail some tests. Possible explanations:
problems with local Perl installation, network problems, previously
undetected bug in Bioperl, flawed test script, problems with CGI script
using for sequence retrieval at public database, and so on. Remember that
there are over 800 modules in Bioperl and the test suite is running more
than 12000 individual tests, a few failed tests may not affect your usage
of Bioperl.
If you decide that the failed tests will not affect how you intend to use
Bioperl and you'd like to install anyway do:
>./Build install
This is what most experienced Bioperl users would do. However, if you're
concerned about a failed test and need assistance or advice then contact
bioperl-l@bioperl.org.
To './Build install' you need write permission in the perl5/site_perl/source
area (or similar, depending on your environment). Usually this will require
you becoming root, so you will want to talk to your systems manager if you
don't have the necessary privileges.
It is also straightforward to install the package outside of the this
standard Perl5 location. See INSTALLING BIOPERL IN A PERSONAL MODULE
AREA, below.
WHERE ARE THE MAN PAGES?
When using Makefile.PL (no longer covered in this documentation), we had
to disable the automatic creation of man pages because this step was
triggering a "line too long" error on some OSs due to shell constraints.
If you want man pages installed use the Build.PL installation process
discussed above.
EXTERNAL PROGRAMS
Bioperl can interface with some external programs for executing analyses.
These include clustalw and t_coffee for Multiple Sequence Alignment
(Bio::Tools::Run::Alignment::Clustalw and
Bio::Tools::Run::Alignment::TCoffee) and blastall, blastpgp, and
bl2seq for BLAST analyses (Bio::Tools::Run::StandAloneBlast), and
to all the programs in the EMBOSS suite (Bio::Factory::EMBOSS).
Environment Variables
Some modules which run external programs need certain environment
variables set. If you do not have a local copy of the specific executable
you do not need to set these variables. Additionally the modules will
attempt to locate the specific applications in your runtime PATH variable.
You may also need to set an environment variable to tell BioPerl about
your network configuration if your site uses a firewall.
Setting environment variables on unix means adding lines like the
following to your shell *rc file.
For bash or sh:
export BLASTDIR=/data1/blast
For csh or tcsh:
setenv BLASTDIR /data1/blast
Some environment variables include:
+------------------------------------------------------------------------+
| Env. Variable | Description |
|---------------+--------------------------------------------------------|
| |Specifies where the NCBI blastall, blastpgp, bl2seq, |
|BLASTDIR |etc.. are located. A 'data' directory could also be |
| |present in this directory as well, you could put your |
| |blastable databases here. |
|---------------+--------------------------------------------------------|
| |If one does not want to locate the data dir within the |
|BLASTDATADIR or|same dir as where the BLASTDIR variable points, a |
|BLASTDB |BLASTDATADIR or BLASTDB variable can be set to point to |
| |a dir where BLAST database indexes are located. |
|---------------+--------------------------------------------------------|
|BLASTMAT |The directory containing the substitution matrices such |
| |as BLOSUM62. |
|---------------+--------------------------------------------------------|
|CLUSTALDIR |The directory where the clustalw executable is located. |
|---------------+--------------------------------------------------------|
|TCOFFEEDIR |The directory where the t_coffee executable is located. |
|---------------+--------------------------------------------------------|
| |If you access the internet via a proxy server then you |
| |can tell the Bioperl modules which require network |
| |access about this by using the http_proxy environment |
|http_proxy |variable. The value set includes the proxy address and |
| |the port, with optional username/password for |
| |authentication purposes |
+------------------------------------------------------------------------+
INSTALLING BIOPERL SCRIPTS
Bioperl comes with a set of production-quality scripts that are
kept in the scripts/ directory. You can install these scripts if you'd
like, simply answer the questions on 'perl Build.PL'.
The installation directory can be specified by
perl Build.PL
./Build install --install_path script=/foo/scripts
By default they install to /usr/bin or similar, depending on platform.
INSTALLING BIOPERL IN A PERSONAL MODULE AREA
If you lack permission to install perl modules into the standard
site_perl/ system area you can configure Bioperl to install itself
anywhere you choose. Ideally this would be a personal perl directory or
standard place where you plan to put all your 'local' or personal perl
modules.
Example:
>perl Build.PL --install_base /home/users/dag
>./Build test
>./Build install
This tells perl to install all the various parts of bioperl in the desired
place, e.g. creating:
/home/users/dag/lib/perl5/Bio/Perl.pm
Then in your Bioperl script you would write:
use lib "/home/users/dag/lib/perl5/";
use Bio::Perl;
For more information on these sorts of custom installs see the documentation
for Module::Build.
If you are used to using something like:
>perl Makefile.PL PREFIX=/home/users/dag
You can get similar behaviour by using this instead:
>perl Build.PL --prefix /home/users/dag
For more information, see Module::Build::Cookbook documentation for
Installing_in_the_same_location_as_ExtUtils::MakeMaker
You can also use CPAN to install accessory modules in your local
directory. First enter the CPAN shell, then set the arguments for the
command "perl Makefile.PL", like this:
>perl -e shell -MCPAN
cpan>o conf makepl_arg LIB=/home/users/dag/My_Local_Perl_Modules
INSTALLING BIOPERL MODULES THE HARD WAY
As a last resort, you can simply copy all files in Bio/ to any directory
in which you have write privileges. This is generally NOT recommended
since some modules may require special configuration (currently none do,
but don't rely on this).
You will need to set "use lib '/path/to/my/bioperl/modules';" in your perl
scripts so that you can access these modules if they are not installed in
the standard site_perl/ location. See above for an example.
To get manpage documentation to work correctly you will have to
configure man so that it looks in the proper directory. On most systems
this will just involve adding an additional directory to your $MANPATH
environment variable.
The installation of the Compile directory can be similarly redirected, but
execute the make commands from the Compile/SW directory.
If all else fails and you are unable to access the perl distribution
directories, ask your system administrator to place the files there for
you. You can always execute perl scripts in the same directory as the
location of the modules (Bio/ in the distribution) since perl always
checks the current working directory when looking for modules.
USING MODULES NOT INSTALLED IN THE STANDARD LOCATION
You can explicitly tell perl where to look for modules by using the
Lib module which comes standard with perl.
Example:
#!/usr/bin/perl
use lib "/home/users/dag/lib/perl5/";
use Bio::Perl;
#<...insert whizzy perl code here...>
Or, you can set the environmental variable PERL5LIB:
csh or tcsh:
setenv PERL5LIB /home/users/dag/lib/perl5/
bash or sh:
export PERL5LIB=/home/users/dag/lib/perl5/
THE TEST SYSTEM
The Bioperl test system is located in the t/ directory and is
automatically run whenever you execute the './Build test' command
(having previously run 'Perl Build.PL'; if you have already installed
Bioperl answer 'no' to script installation to get nicer test output
later).
Alternatively if you want to investigate the behavior of a specific test
such as the Seq test you would type:
>./Build test --test_files t/Seq.t --verbose
The ./ ensures you are using the Build script in the current directory to
make sure you are testing the modules in this directory not ones
installed elsewhere. The --test_files arguement can be used multiple times
to try a set of test scripts in one go. The --verbose arguement outputs
the detailed test results, instead of just the summary you see during
'./Build test'.
If you are trying to learn how to use a module, often the test suite is a
good place to look. All good extreme programmers try and write a test
BEFORE they write the module to insure that their module behaves the way
they expect. You'll notice some 'ok' and 'skip' commands in a test, this
is part of the Perl test suite that signifies a passed test with an 'ok
N', where N is the test number. Alternatively you can tell Perl to skip
tests. This is useful when, for example, your test detects that the
network is not present and thus should skip, not fail, any tests that
require a network connection.
BUILDING THE OPTIONAL bioperl-ext PACKAGE
The bioperl-ext package contains C code and XS extensions for
various alignment and trace file modules (Bio::Tools::pSW for DNA
Smith-Waterman, Bio::Tools::dpAlign for protein Smith-Waterman,
Bio::SearchDist for EVD fitting of extreme value,
Bio::SeqIO::staden).
This Installation works out-of-the box for all platforms except BSD
and Solaris boxes. For other platforms skip this next paragraph.
CONFIGURING for BSD and Solaris boxes
You should add the line -fPIC to the CFLAGS line in
Compile/SW/libs/makefile. This makes the compile generate position
independent code, which is required for these architectures. In addition,
on some Solaris boxes, the generated Makefile does not make the correct
-fPIC/-fpic flags for the C compiler that is used. This requires manual
editing of the generated Makefile to switch case. Try it out once, and if
you get errors, try editing the -fpic line
INSTALLATION
Move to the directory bioperl-ext. This is available as a separate package
code and XS extension for the bp_sw module is held and execute these
commands: (possibly after making the change for BSD and Solaris, as
detailed above)
perl Makefile.PL # makes the system specific makefile
make # builds all the libaries
make test # runs a short test
make install # installs the package correctly.
This should install the compiled extension. The Bio::Tools::pSW
module will work cleanly now.