NAME

primer_designer.pl -- command-line interface Bio::PrimerDesigner

SYNOPSIS

  ./primer_designer.pl [options] [dna_sequence or file]

Options:

-p|--program   Program (default "primer3")
-n|--number    Number of primer sets to return (default "5")
-b|--binary    Path to binary executable (default "/usr/local/bin")
-m|--method    "local" or "remote" (default "local")
-u|--url       URL of remote primer3/e-PCR system (default 
                 "aceserver.biotech.ubc.ca/cgi-bin/primer_designer.cgi")
--list-aliases Print alias list to primer3 input options and exit
--list-params  Print a list of primer3/e-PCR input options and exit
-h|--help      Print help and exit

DESCRIPTION

This script tests/demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR. It can be used with a local installation of the unix binaries or remotely via an HTTP/CGI request.

AUTHOR

Copyright (C) 2003-2009 Sheldon McKay <mckays@cshl.edu>, Ken Youens-Clark <kclark@cpan.org>.

SEE ALSO

Bio::PrimerDesigner.

LICENSE

This program is free software; you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation; version 3 or any later version.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program; if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.